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murAB [2019-02-19 19:13:55]

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Locus

BSU37100

Isoelectric point

5.83

Molecular weight

45.85 kDa

Protein length

429 aa Sequence Blast

Gene length

1290 bp Sequence Blast

Function

peptidoglycan precursor biosynthesis

Product

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Essential

E.C.

2.5.1.7

Synonyms

murZ, lssF, rev-4

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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Categories containing this gene/protein

Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of peptidoglycan

Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of peptidoglycan

Gene

Coordinates

3,806,086 3,807,375

The protein

Catalyzed reaction/ biological activity

Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)

Protein family

MurA subfamily (according to Swiss-Prot)

Paralogous protein(s)

MurAA

Structure

3SG1 (from B. anthracis, 50% identity, 81% similarity)

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

Genes

murAB-glpX

Description

PubMed

Regulation

constitutive PubMed

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Biological materials

Mutant

BKE37100 (murAB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAGTCTCCTTCCATA, downstream forward: _UP4_TAATGGTTTCTTGTGAGAGG

BKK37100 (murAB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAGTCTCCTTCCATA, downstream forward: _UP4_TAATGGTTTCTTGTGAGAGG

Expression vectors

pGP2595: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jrg Stlke's lab

References

Reviews

Original publications